In order to increase the juice yield of black rice, the medium temperature α-amylase treatment with single factor and orthogonal experiments was used. The results showed that the optimal conditions were following: cooking time 10 min, pasting time 20 min, dosage of enzyme 1%, enzymolysis temperature 70 ℃. Under these conditions, the highest juice yield of black rice was 80.25%, which was increased by 26.88% compared with the control group. It showed that this method can increase the juice yield of black rice with shorter treatment time.