大米中的维生素B1经酸解、酶解、氧化、萃取后，采用高效液相色谱法，经过C18色谱柱，以甲醇∶0.05 mol/L乙酸钠溶液（pH=4.5）=35∶65作流动相，用荧光检测器进行检测。结果表明，维生素B1在0~0.20 μg/mL的范围内具有较好的线性关系，检出限为0.65 ng/mL，三个浓度水平的加标回收率均在95%以上，6次平行试验的相对标准偏差为2.93%，t检验表明本方法与GB/T 7628—2008法之间无显著性差异。
The vitamin B1 in rice was extracted by acid hydrolysis, enzymatic hydrolysis and oxidation. A C18 column was used for separation and elution. The mobile phase was methanol and sodium acetate (0.05 mol/L, pH=4.5, 35∶65). The vitamin B1 was detected by fluorescence detector quantificationally. The results showed that vitamin B1 had a good linear relationship in the concentration range of 0 ~ 0.20 μg/mL. The detection limit was 0.65 ng/mL. The recoveries of the three levels were above 95%. The relative standard deviations were 2.93% (n=6). There was no conspicuous difference between the result by this method and the result by the method described in GB/T 7628-2008.