粮油食品科技

中国科技核心期刊

高效液相色谱法测定大米中的维生素B1
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Determination of vitamin B1 in rice by high performance liquid chromatography
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    大米中的维生素B1经酸解、酶解、氧化、萃取后,采用高效液相色谱法,经过C18色谱柱,以甲醇∶0.05 mol/L乙酸钠溶液(pH=4.5)=35∶65作流动相,用荧光检测器进行检测。结果表明,维生素B1在0~0.20 μg/mL的范围内具有较好的线性关系,检出限为0.65 ng/mL,三个浓度水平的加标回收率均在95%以上,6次平行试验的相对标准偏差为2.93%,t检验表明本方法与GB/T 7628—2008法之间无显著性差异。

    Abstract:

    The vitamin B1 in rice was extracted by acid hydrolysis, enzymatic hydrolysis and oxidation. A C18 column was used for separation and elution. The mobile phase was methanol and sodium acetate (0.05 mol/L, pH=4.5, 35∶65). The vitamin B1 was detected by fluorescence detector quantificationally. The results showed that vitamin B1 had a good linear relationship in the concentration range of 0 ~ 0.20 μg/mL. The detection limit was 0.65 ng/mL. The recoveries of the three levels were above 95%. The relative standard deviations were 2.93% (n=6). There was no conspicuous difference between the result by this method and the result by the method described in GB/T 7628-2008.

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曹阳,刘美辰.高效液相色谱法测定大米中的维生素B1[J].粮油食品科技,2014,22(6):71-73.

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  • 在线发布日期: 2014-12-29
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