The peanut protein isolated film was treated by physical treatments including heating, ultrasonic and microwave to observe their effects on mechanical strength, moisture barrier property and light transmission property. The results showed that the tensile strength and light transmission of the films were increased significantly by the treatments of heating for 30 min at 75 ℃, or ultrasound with the output power 1 000 W for 3 min or microwave with the output power 500 W for 2 min. The moisture barrier property of the films increased after heat denaturation and ultrasonic treatment, while microwave treatment had a little influence on it.