A closed microwave digestion- ICP-MS method was established to determine simultaneously As, Pb, Hg, Mg, Al content in nuts and fruit and vegetable seed kernel. The samples were digested with microwave digestion instrument and analyzed by inductively coupled plasma mass spectrometer, 72Ge as an internal standard of As, 209Bi as an internal standard of Pb and Hg, 45Sc as the internal standard of Mg and Al to control the dynamic drift of the analytical signals, As, Pb, Hg, Mg, Al content in nuts and fruit and vegetable seed kernel were determined simultaneously. The results showed that the recoveries of As, Pb, Hg, Mg, Al between 95%~105%; the detection limits of them were 0.005 mg/Kg, 0.005 mg/Kg, 0.001 mg/Kg, 0.01 mg/Kg, 0.005 mg/Kg; all of the linear correlation coefficient more than 0.999 9; RSD less than 2%. The results, recovery, detection limit, accuracy and precision, met the requirements of the determination of As, Pb, Hg, Mg, Al content in nuts and fruit and vegetable seed kernels, which provided a basis for quality standards of nuts and fruit and vegetable seed kernel.