The method of urea adduction fractionation was used to purify the oleic acid and linoleic acid in camellia oil. Saturated fatty acid ethyl esters, ethyl oleate and ethyl linoleate were separated by stepwise cooling. The effect of crystallization temperature in the first and the second stage and urea amount on the oleic acid yield was reviewed. The optimal technical parameter were the first stage crystallization temperature was 5 ℃, and the second one was -10 ℃, the urea amount was 1∶1. The yield of oleic acid was 86.19% and linoleic acid 82.17%. The result showed that the first stage crystallization temperature was the main factor to affect purification. The separation and purification of oleic acid and linoleic acid were realized, which settled a good foundation for the application of camellia oil in the food and pharmaceuticals.