The application and the feasibility of PCR technology in detection of chicken and cattle derived ingredients in edible oil were studied. The result showed that chicken derived ingredient in the sample 5 and 6 was negative, and cattle derived ingredient in the sample 4 and 5 was negative. But chicken and cattle derived ingredients in the sample 1,2 and 3 were positive, and chicken derived ingredient in the sample 4 was positive while cattle derived ingredient in the sample 6 positive.All the results showed that it is feasible to detect animal derived ingredient in edible oil by PCR technology.