Abstract:The effect of four different macroporous absorption resins on the extraction of proanthocyanidin from peanut seed coat was compared. The optimum macroporous adsorption resin was screened out which was AB-8. The optimal conditions were: concentration of the sample was 2.5 mg/mL, loading flow rate was 0.5 mL/min. The proanthocyanidin was eluted with ethanol concentration of 20%, 30%, and 50%, and three levels of the liquid, PSPP1, PSPP2, and PSPP3, were obtained with the proanthocyanidin content 75.26%, 84.50%, and 90.18% respectively. The thin layer chromatography analysis showed that the purified liquid had three bands, initial inference as monomer, mixture of oligomers, and high polymers.