Wheat gluten was hydrolyzed by alkaline protease to obtain the peptide with high content of gln and the technics was optimized by single-factor and orthogonal experiment. The effective content of gln in peptide was determined by amino acid analyzer. The results indicated that the optimal parameters to hydrolyze wheat gluten were as follow: pH 8.0, temperature 70℃, enzyme concentration E/S 4%, substrate concentration 7%, reaction time 150 min. Under such conditions, the effective content of gln could reach 17.65%, account for77.31％ of the total Glx（Glu+ Gln, content 22.83%），TCA-NSI was 77.86％，the protein content was 76.03％, the free amino content was 2.06％. The product had high effective content of gln and peptide.