引用本文:任婷婷,吕双双,李书国.反胶束法萃取玉米胚芽蛋白及其功能性研究[J].粮油食品科技,2014,22(2):80-84.
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反胶束法萃取玉米胚芽蛋白及其功能性研究
任婷婷, 吕双双, 李书国
河北科技大学 生物科学与工程学院,河北 石家庄050018
摘要:
研究了AOT/异辛烷反胶束法萃取玉米胚芽蛋白及玉米胚芽蛋白的加工功能性。在实验中分别考察了纤维素酶加酶量、AOT浓度、KCl浓度、缓冲液pH值、W0对玉米胚芽蛋白前萃率的影响,以及萃取时间、KCl浓度、缓冲液pH值对后萃率的影响,确定了前萃的最佳技术条件:加酶量为4 000 IU/g 玉米胚芽、AOT浓度为3 g/50 mL异辛烷、萃取pH 6、KCl浓度0.1 mol/L、W0为25;后萃的最佳技术条件为:KCl浓度为0.5 mol/L、萃取pH 10.5,萃取时间40 min;对玉米胚芽蛋白的部分加工功能性进行研究,结果表明其吸油性(2.9 mL/g)、乳化性(54.5%)、乳化稳定性(86.5%)以及泡沫稳定性(58.3%)都较好,但吸水性和起泡性相对较差,玉米胚芽蛋白不但营养效价高,而且具有较好的加工功能特性,在食品工业中具有应用潜力。
关键词:  反胶束萃取  玉米胚芽  蛋白质提取  加工功能性
DOI:
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Extraction of protein from corn germ by reverse micelle and its functionality
Abstract:
Extraction of protein from corn germ by AOT/isooctane reversed micellar extraction and its processing functionality were studied. The effects of dosage of cellulase, AOT mass concentration, KCl concentration, pH of buffer solution and W0 on the forward extraction rate of the protein, and the effects of extraction time, KCl concentration, pH of buffer solution on the backward extraction rate were investigated. The optimum forward extraction conditions were determined: dosage of cellulase 4 000 IU/g corn germ, AOT mass concentration 3 g/50 mL isooctane, pH 6, KCl concentration 0.1 mol/L, W0 25. The optimum backward extraction conditions were as follows: KCl concentration 0.5 mol/L, pH 10.5, and extraction time 40 min. Some processing functionality of corn germ protein was studied. The result showed that oil absorptivity (2.9 mL/g), emulsifiability (54.5%),emulsion stability (86.5%) and foam stability (58.3%) were good. But water absorptivity and foamability was slightly poorer than rice protein. Corn germ protein has higher nutritive value and perfect processing functionality; therefore it can be used in food industry.
Key words:  reversed micellar extraction  corn germ  protein extraction  processing functionality