The temperature changes of grain piles during rice storage in high-temperature and high-humidity grain storage areas were studied under the air-conditioning temperature control mode with a temperature of 22 ℃ and a daily opening time of 13 h and a temperature of 24 ℃ and a daily opening time of 24 h. The changes of rice moisture and fatty acid values ​​before and after temperature-controlled storage were compared, and the differences in temperature changes in each layer in the horizontal direction of grain piles at different depths were analyzed. The results showed that the average temperature and the maximum grain temperature of the grain pile increased slowly under different air-conditioning operation modes. Air-conditioned temperature-controlled grain storage had a greater impact on the surface temperature of the grain pile, but less impact on the middle layer grain temperature. During the air-conditioned temperature-controlled storage period, the lower layer grain temperature may be affected by the floor temperature. The temperature control mode that lowered the temperature control temperature but shortened the temperature control time was more economical and efficient. The temperature control mode of 22 ℃ + 13 h could save about 12.4% of electricity costs compared with the temperature control mode of 24 ℃ + 24 h. At the same time, the control effect of the warehouse temperature, surface grain temperature and average grain temperature of the grain pile with a lower basic grain temperature was more obvious, and the basic grain temperature of the grain pile played an important role in the implementation of temperature-controlled grain storage technology.