The innovative development of green technology is the permanent scientific research topic in the field of grain storage, and the controlled atmosphere is one of the green grain storage technologies. This paper carried out the research on the storage technology of high-quality indica rice in the fifth ecological area of grain storage (medium temperature and high humidity) by air-regulating agent. The concentration of oxygen, temperature and relative humidity of the storage environment were monitored, and the quality of high-quality indica rice was also evaluated. The results showed that the technology of air-regulating could effectively delay the quality deterioration of rice. Compared with the controlled group, the experiment group can maintain the concentration of oxygen in the confined space below 0.1% and the relative humidity at 70% during the 5-month storage period. The moisture content of the controlled atmosphere group was 0.98% higher than that of the controlled group. The fatty acid value was 1.34 mg (KOH)/100 g lower than that of the controlled group, while the freshness was 7 points higher than that of the controlled group. The above results showed that the air-regulating agent can adjust and control the oxygen concentration and relative humidity in the confined space according to the demand, which could achieve the purpose of preserving the quality and freshness of grain. It is a green, low-carbon and fresh-reserving new grain storage technology.