This paper aims to investigate the key points for olive oil total migration test for multilayer laminated films. The samples were made into 1.20 dm2 specimens. The tests of total migration in olive oil were carried out according to the standard GB31604.8—2021. It was found that the results of moisture- sensitive determination for the same material samples were various. The results of olive oil total migration differed greatly between conditioned and unconditioned moisture-sensitive samples. The test results of the volatiles measurement of the specimens also affected the final value of the total migration. Concurrently, the results of the volatiles measurement of the specimens of different multilayer composite films were different. Due to the different materials, the laminate membrane had different adsorption capacity for olive oil. The result determinations were affected by the completeness of olive oil extraction. Therefore, the test results of total migration in olive oil should fully consider the steps of specimen moisture conditioning, volatile matter determination and the number of olive oil extraction to ensure the accuracy of test results.