A closed gas-up reactor was selected for self-cultured Chlorella pyrenoidosa, the ventilation of carbon dioxide from carbon source obtained by univariate optimization was 2%, and the sodium nitrate of nitrogen source was 10 g/L. More suitable medium components were obtained through the uniform design of Chlorella pyrenoidosa culture medium: K2HPO4 of 0.6 g/L, MgSO4 of 0.2 g/L, NaNO3 of 11 g/L, K2SO4 of 0.8 g/L, ammonium ferric citrate of 0.006 g/L, CaCl2 of 0.05 g/L, vitaminB1 of 0.11 g/L, EDTANa2 of 0.01 g/L, Na2WO4 of 0.08×10–3 g/L, NiCl2 of 0.02×10–3 g/L; In the experiment, a suitable light source with intermediate white light and surrounding colored light was used, and the oxygen was maintained at about 20% with an oxygen scavenger, under which 8.648 g/L dry cell was obtained finally. The protein content of Chlorella pyrenoidosa is as high as 58 g/100 g, and the amino acid and mineral elements of Chlorella pyrenoidosa is also rich by analysis. We studied the replacement of wormwood powder with Chlorella pyrenoidosa, and produced Chlorella pyrenoidosa qingtuan.