In this paper, the protective effect of a botanical beverage with pueraria extract and Hovenia dulcis Thunb. extract on acute alcoholic liver injury in mice was investigated. The acute alcoholic liver injury model was established by a one-time gavage of 50% alcohol, and then the relevant biochemical indicators and liver tissue morphology were detected to evaluate the hepatoprotective effect of the botanical beverage. Compared with the model control group, the middle-dose of plant beverage with pueraria extract and Hovenia dulcis Thunb. extract can significantly reduce the levels of ALT, AST, TC, TG and LDL in the serum of mice with acute alcoholic liver injury to (19.17±6.22) U/L, (14.81±4.61) U/L, (4.77±0.33) mmol/L, (1.92±0.24) mmol//L, (0.22±0.09) mmol/L, increase the activities of SOD, GSH-Px and GSH in liver, and decrease MDA content to (0.80±0.09) nmol/mg pro. This plant drink can also significantly improve the pathological changes of liver tissue to a certain extent, and reduce steatosis and inflammatory cell infiltration. The experimental results showed that the plant beverage with pueraria extract and Hovenia dulcis Thunb. extract had a certain protective effect on the liver tissue of mice after acute alcohol exposure.