According to the requirements of GB/T 15000—2008 《Directives for the work of reference materials preparation》, the method of preparing reference materials for determination of wheat flour quality curve- stability time is established. High quality wheat flour, fully mixed and packed and without any additives, is used as raw materials. The uniformity and stability test and fixed value analysis of the stability time of the samples are carried out by Farinograph. The results show that the reference materials have good uniformity and stability under the storage condition with the temperature of 0 ℃ to 10 ℃ for 24 months. A cooperative certification is conducted with 11 qualified laboratories. The certification result of the reference material is 10.3 min and the expanded uncertainty is 0.6. The reference material conform to the technical requirements of the national standard sample development guidelines and can be used for the verification of wheat flour stability time test method and Farinograph, and it also has applications in the level assessment of laboratory personnel and the quality control of the detection process.