Beneficial mineral elements are one of the indispensable nutrients in human body. With the adjustment of diet structure, people’s demand for mineral elements in wheat is becoming stronger and stronger. Therefore, screening wheat germplasm resources with low heavy metal content and high beneficial mineral elements has important theoretical and practical significance for cultivating new wheat varieties with high nutrient mineral elements and low heavy metal content and ensuring the quality and safety of agricultural products. In this study, 205 wheat germplasms from 2 areas were collected to determine the content of mineral elements and screen germplasm resources with high beneficial mineral elements and low heavy metals under four environments in three years. The results showed that the contents of mineral elements were different in different environments and different wheat varieties. In general, the content of each element showed continuous distribution, which was a typical quantitative character. The contents of calcium, manganese, copper, zinc and selenium in wheat grain in Dezhou point were significantly higher than those in Tai'an, while the contents of heavy metals in Tai’an were lower than those in De Zhou. A total of 38 varieties (lines) with high content of beneficial mineral elements were selected, such as B111 with the highest content of calcium, and the variety with the highest iron content, such as B189. There were 24 varieties (lines) with high contents of various beneficial mineral elements, such as B45, B38 and B21, whose grains contained 4 kinds of high contents of beneficial mineral elements. A total of 33 varieties (lines) with high beneficial mineral elements and low harmful mineral elements contents were identified, such as B125 with higher content of Ca, Fe, Cu and lower Pb.