To establish a method for the determination of phthalic acid esters in Peanut oil, Peanut oil samples were extracted with n-hexane saturated acetonitrile, purified by 25 mg PSA and 25 mg C18 and detected by GC-MS selected ion monitoring (SIM) mode detection, external standard method quantitative. The standard solution of 17 phthalates showed a good linearity in the concentration range of 0.02~3.0 mg/L, and the correlation coefficient (r) is above 0.999, the detection limit (LOD) was 0.01 mg/kg to 0.02 mg/kg, and the quantitative limit (LQD) was 0.03 mg/kg to 0.05 mg/kg. the recovery of standard addition was 92.47% to 108.72%, and the relative standard deviation was 1.10% to 5.94%.The method is simple, rapid, sensitive and accurate. and can be used for the determination of 17 phthalates in Peanut oils.