Seabuckthorn seed was used as raw material to prepare biochar adsorbent at 300, 400 and 500 ℃, which were named BC300, BC400 and BC500, whose effect on removing phenol was detected. The results of adsorption experiments showed that the preparation temperature of biochar significantly affects its adsorption effect on phenol. The adsorption capacity of three kinds of biochar was BC500> BC400> BC300. In addition，the initial concentration of phenol, the temperature and time of adsorption can also affect the adsorption result. When the initial concentration of phenol was 20 mg/L and the adsorption temperature was 45 ℃, the removal rate of phenol was up to 92.1% by BC500. The sorption isotherm of phenol in biochar conform to Langmuir mode and Freundlich mode. The results showed the method of preparing biochar adsorbent and the optimum condition of phenol removal from aquous solution by seabuckthorn seed dregs, thus providing a theoretical basis for removal of organic pollutants such as phenol.