The optimal germination conditions of sesame and the change of main antioxidant composition in sesame during germination were researched with Vitamin E and sesame lignan as evaluation indexes by single-factor, orthogonal array design and correlation analysis methods. The results showed that the optimal germination conditions of sesame was: the ratio of sesame to water was 50∶50 g/g, temperature 35 ℃，and germination time 8 h. Under the optimized conditions, the content of sesame lignan reached to 748.83 mg/100 g, which increased by 5.39% compared with before. Correlation analysis showed that sesame lignan was positively correlated with Vitamin E. The content of Vitamin E reached to 546.3 mg/kg, which increased by 4.50% compared with before.