The composition of 6# residual solvent in soybean crude oil and their area percentages were analyzed and determined by gas chromatograph combined with gas chromatography-mass spectrometry, which indicated that the difference of the ratio between each batches of the soybean crude oil was significant. The fresh extracted soybean oil was used as the basis material to compound standard mixed solution of component to analyze and calculate the relative mass correction factor between 0.34～0.81. The residual solvents in the different batches of the samples can be precisely measured by using different component as standard solution and applying external standard method to quantitative each component then summation calculation, and verified by paired t-test.