The embedding technology of zearalenone degrading enzyme ZLHY6 was studied. The effects of temperature and pH on the embedding enzyme ZLHY6 were investigated and the relative enzyme activity was used to evaluate the stability of enzyme activity of ZLHY6. The results showed that the optimal reaction temperature was 35℃, and the enzyme retained substantial activity after the enzyme incubation at 50℃ for 4h. The optimum pH was 8.0, and 80% enzyme activity was retained after being at pH 5.0 for 2h. In addition,the results of in vitro simulation test of porcinegastrointestinal fluid showed that 61% enzyme activity of the embedding enzyme was retained after being digested bygastric fluid for 4h and intestinal fluid for 2.5h. The tolerance of zearalenone degrading enzyme ZLHY6 against temperature, pH and simulative gastrointestinal fluid was improved after being embedded.