The pretreatment conditions for the determination of amylose in national standard method was improved by ultrasonic degreasing and constant temperature drying. The content of amylose in rice was detected by amylose detector and verified by the national standard method. The rapid detection method for amylose was established based on colorimetric method. The optimum condition of ultrasonic degreasing was determined by the experiment: water temperature 50℃, and the time 50min; the optimum condition of constant temperature drying: temperature 105℃, drying time 80min.T test showed that there was no significant difference between the results of the two detection methods. This method overcame the human error to agreat extent, shortened the experiment time and improved the accuracy, repeatability and reproducibility of the experiment.