GB 2760—2014 provides that titanium dioxide should not be used in wheat flour and its products. Therefore, it is critical to judge whether adding titanium dioxide or not into wheat flour and its products by the background value of titanium dioxide. The content of titanium dioxide in wheat flourground in laboratory, wheat flour and its products bought from market were determined by diantipyrylmethane colorimetry ingB 5009.246—2016 and inductively coupled plasma-mass spectrometry (ICP-MS) ingB 5009.268—2016 in order to provide scientific principles for the background value of titanium dioxide in wheat flour, which had high practical reference value. The results showed that: all of the 60 portions of wheat flourground in laboratory had titanium dioxide with average value as 2.98mg/kg, the amplitude range of 1.42mg/kg～5.42mg/kg, normal distribution, the confidence interval was 2.74mg/kg～3.20mg/kg while confidence probability was 95%. According to the principle of statistics the 20 portions of wheat flour and its products bought from market were tested by ICP-MS method. It is feasible to use 6mg/kg as the detection limit of titanium dioxide, which can reduce the occurrence of misjudgment.