Flavonoids were extracted from perilla oil meal by microwave-assisted extraction.The results showed that the optimum parameters were as followings:the ratio of solid to liquid was 1:20g/mL,the ethanol concentration was 60%,the microwave power was 500W and the microwave time was 60s.According to the above conditions,the extraction rate could reach 3.04%.Compared with traditional extraction method by ethanol,the extraction rate increased 54.31%.Concentration of the extracts in the range of 0.05～0.3mg/mL had a remarkable dose-effect relationship with the antioxidant activity.When the concentration of the flavonoids from the perilla oil meal was 0.3mg/mL,the clearance rate of the hydroxyl radicals and O2-·were up to 75%,77%.The studies provided a basis for the development and utilization on the flavonoids of perilla oil meal.