In order to explore the best extraction technology of pomegranate peel polyphenols by ethanol under different conditions, the influences of ethanol concentration, solid-liquid ratio, extraction time and extraction temperature on the extraction yield were researched by the single factor experiments and response surface analysis. The results showed that: the extraction yield of pomegranate peel polyphenols was 14.09% under the condition of taking 60% ethanol solution as extractant, with solid-liquid ratio 1∶25g/mL, at 30℃ extracting for 60min. The results of bacteriostatic experiment showed that theminimum bacteriostasis concentration of pomegranate peel polyphenols against staphylococcus aureus and escherichia coli, was 3 mg/mL,while the diameters of the inhibition zones were 9.36mm and 8.64mm respectively.