The study was aimed to screen the strain for efficiently detoxifying zearalenone(ZEN). Two strains named Fu2-3 and Fu41-3 withgood performance in scavenging ZEN were screened out by 96-well plate high throughput screening among 135 soil samples collected from different areas where were highly infected bygibberellic disease. The strains were preliminarily identified as Bacillus amyloliquefaciens and Bacillus tequilensis based on 16S rRNAgene sequencing. The preliminary study on their detoxification mechanism showed that they were able to adsorb ZEN, but the main detoxification was bio-degradation. The result of BLYES assay indicated that the detoxification system of strain Fu41-3 still contained considerable estrogen toxicity,while strain Fu2-3 did not, by which ZEN would be transformed into metabolite with extremely low estrogenic toxicity. The two strains were isolated through screening, which provides theoretical evidence for bio-detoxification of ZEN.