A kind of lactone tofu was prepared by using gordon euryale seed and soybean as main raw materials and adding delta-gluconolactone(GDL) as tofu coagulant. The optimum technology parameters were acquired by single factor and orthogonal experiments, while sensory evaluation and dry base yield of the lactone tofu as indicators. The optimum parameters were the ratio of soybean to water 1∶5g/mL, the ratio of seed to water 1∶15g/mL, the ratio of soybean milk to seed slurry 8∶2mL/mL, additive amount of GDL 0.30%, at 85℃ water bath for 40min till tofu coagulated. The product was acquired by cooling and setting. It was beige white, and had soybean flavor and gordon euryale seed flavor.