An analytical method based on solid phase extraction-high performance liquid chromatography was established for the determination of six oryzanol compositions and total oryzanol content in rice bran oil. The samples were purified by diol-silica gel series SPE column. The standard sample (γ-Oryzanol) was used for quantification. The chromatographic column was Agilent ZORBAX Eclipse Plus C18（250mm×4.6mm，5μm）and the mobile phase was acetonitrile with 1% acetic acid∶isopropanol =95∶5. The wavelength of UV was 321 nm.The average recoveries of six compositions and total content were in the range of 95.6%～105.7% and 97.8%～100.2% with relative standard deviations of 0.1%～4.6% and 0.7%～3.4%.The method was suitable for determination of oryzanol composition and content in rice bran oil, which provided technical support for further research of oryzanol.