Saccharomyces cerevisiae induced by UV mutagenesis to breeding the strains of high fermentation rate and alcohol production are investigated.Regarded fatality rate and percentage of plus mutants as main criterion，ethanol fermentation performance of mutant as reference，the proper condition of UV mutagenesis experimented by single factor and orthogonal experiment are as follows：UV radiation time for 10 min by ultraviolet lamp of 20w，distance from the lamp to sample at 9cm.And with the original strain F1 as control，physiological comprehensive performance tests of the seven mutant stains are conducted.The results are shown that an excellent positive mutated S.cerevisiae F4 is obtained，which has a high loss of carbon dioxide weight，low amount of residual sugar，superior production of ethanol and esters.The cell of this mutated strain F4 is full，its shape is oval，and its color is ivory-white or cream.It could be applied in the wine of purple sweet potato，of which the fermentation rate and alcohol production are high，the alcohol degree of fermentation broth can reach at 11.08%，the aroma is strong.So the potential industrial production of this mutated strain is well.