Cottonseed meal is an important protein feed resource，however the existence of free gossypol affects its application in animal husbandry.In this paper，a strain ST-141，which could remove more than 90% of free gossypol in the liquid fermentation was isolated and identified as Bacillus subtilis through 16S rDNA sequence analysis and Biolog microbial identification.Then the ST-141，mixed with the strain of Saccharomyces cerevisiae N5，were used for the solid state fermentation of cottonseed meal.After incubated at 30 ℃ for 48 h，the removal rate of free gossypol reached 48.5%，and the contents of crude protein，total amino acid and essential amino acid were also increased by 19%，22.2% and 28.4% respectively，showing high application potential.