Low field nuclear magnetic resonance（NMR）technology was used to resrarch water relaxation characteristic under different water content gradient in tempering process，the high quality wheat zhengmai 366 as raw material in this paper.The results show that：T21，A21 was positively correlation with water content gradient and showed a trend of rising in tempering process；T22，T23，A22 and A23 are present a trend of first raising and faling after，varity parameters change have a positive correlation with moisture gradient，while T23，A23 have no significant correlation between moisture gradient.Water addition amounts had a significant effect on the state of water activity and content in wheat tempering process.Moreover，compared with the infiuence of water addition amounts to each component water total content，the infiuence of water addition amounts to each state moisture degree of freedom was greater.