The electrical stimulation on frog gastrocnemius to simulate peripheral fatigue was established. The antifatigue effect of purple sweet potato anthocyanins(PSPA) was studied, as well as the difference of anti-fatigue effect among different varieties of PSPA. Three groups with different concentrations（0.03、0.06、0.12 mg/mL）of PSPA were set, so did treatment time（5、10、15、20 min）. The control group was exposed to solutions containing Ringer's solution. The peripheral fatigue by electrical stimulation was recorded as skeletal muscle contraction curve. The anti-fatigue effects were compared among different varieties of PSPA with mass concentrations of 0.06 mg/mL for 10 min. The results showed that PSPA significantly delay the fatigue of the gastrocnemius (P<0.05), and at the same concentration, the longer the processed time, the more significant the delay of gastrocnemius fatigue (P<0.05). In the 5 min, extensions of time of the groups with concentration of 0.12 mg/mL and 0.06 mg/mL were 2.6 and 1.7 times longer than that with 0.03 mg/mL. The antifatigue effect of the group with concentration of 0.03 mg/mL was 18.19%, while that of 0.06 mg/mL was 38.01%, that of 0.12 mg/mL was 63.23%. PSPA had significant antifatigue effect. Different varieties of PSPA had different antifatigue effect.