The aqueous enzymatic extraction of oil and protein from cold pressed camellia oleifera seed cake was optimized by response surface methodology. Taking enzyme dosage, temperature, pH and hydrolysis time as influencing factor, the extraction yields of oil and protein as response value, a mathematic model was established using Box-Behnken experimental design．The result showed that the model was statistically significant and allowed accurate prediction of extraction yields of oil and protein under various conditions．The optimal technical condition was: ratio of solid to liquid 1∶5，enzyme dosage 1.6%, pH 8, temperature 60 ℃ and hydrolysis time 4.5 h. Under the optimized conditions，the extraction yields of oil and protein were 81.42% and 79.36%，respectively.