The relativity and biological absorption ratio characteristic between wheat and its trace copper in the soil was studied , taking Nanyang color wheat，Zhoumai 16 and Yanzhan 4110 as samples, rapidly determined by fixed-time and concentration direct-reading method. The result showed that the copper content was 2.17~7.81 μg/g in each sample of wheat flour，the highest total content of copper in blue wheat, the lowest total content of copper in Zhoumai 16. The copper content of wheat form Gongyi district was 4.599~7.808 μg/g，above other varieties of wheat background. The copper content was 2.17~4.52 μg/g in wheat form Zhengzhou district，lower than that of the same wheat variety. The biological absorption ratio of wheat was closely related to the varieties and the characteristics of the soil. The biological absorption ratio of Zhoumai 16 was relatively low as 13.06%～22.73%, while that of blue wheat was relatively high as 30.98%～39.81%.