Two bacillus T-246 and T-420, which possess very good degradation effect, have been screened from 217 strains of bacillus, which were cultured in 96-well micro plate with strain and viral co-culture, by primary screen and rescreen. It was confirmed that 15 μg/mL zearalenones (ZEN) could be degraded by these strains within 6 h and 9 h, respectively. These two strains were identified as Bacillus stratosphericus and Bacillus pumilus based on 16S rDNA sequence analysis. In addition, the preliminary study on their degradation mechanism showed that they were able to bind ZEN, but the main effect of scavenging ZEN was due to the degradation activity of extracellular enzyme.