SCIENCE AND TECHNOLOGY OF CEREALS, OILS AND FOODS

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Detection method of Deoxynivalenol in wheat
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    Abstract:

    建立了一种新的小麦脱氧雪腐镰刀菌烯醇(DON)高效液相色谱检测方法。采用聚乙二醇和水对小麦样品进行振荡提取,提取液分别用活性炭、柱层析硅胶、中性氧化铝、酸性氧化铝、碱性氧化铝净化,比较几种吸附剂对小麦DON净化效果。结果表明,用酸性氧化铝净化效果最好,除杂率为66.3%,净化液通过0.22 μm聚醚砜滤膜可直接用于HPLC检测;色谱条件:分离柱为C18柱;流动相为甲醇∶水(20∶80);波长为230 nm。三水平加标平均回收率为91.0%~103.7%,不同污染水平的小麦阳性样品精密度试验相对标准偏差(RSD)为2.3%~8.5%;与免疫亲和柱(IAC)净化方法比较,方法间误差为2.3%~8.4%。本方法具有操作简便、快速、准确、成本低,可满足小麦DON大宗样品定量测定的要求。

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  • Online: September 06,2015
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