The relationship between wheat and trace chromium in soil was studied in order to provide a scientific basis for the breeding of wheat varieties enriched in chromium and development of functional food with rich chromium. With applications of microwave digestion sample, the determination method of chromium content in color wheat, common wheat and soil was established by flow injection chemiluminescence. The results showed that in six varieties of wheat grown in five areas, the chromium content was between 0.126 3~0.451 6μg /g. Different varieties had different content of chromium, chromium content in common purple wheat was higher; the same varieties of wheat planted in different area, the chromium content was different. Wheat planted in Shangqiu the chromium content was higher. Under the same growth environment, different varieties of wheat had different chromium biological absorption ratio, common purple wheat had higher biological absorption ratio, the biological absorption ratio of the common purple wheat planted in Shangqiu was as high as 27.62%, which was an ideal natural raw material to supplement chromium and had a certain cultivation prospects.