The difference of physicochemical properties of wheat protein hydrolysate in 2 different drying conditions was studied in order to provide basic data for the industrial production of wheat protein hydrolysate. Gluten was hydrolyzed by Alcalase, using the fermentation tank as a reactor to obtain the hydrolyzate which was rich in glutamine. Wheat protein hydrolysate was prepared by spray drying or fluidized bed drying. The physicochemical indexes were investigated. The results showed that by spray drying, the content of moisture, protein, peptide, effective glutamine and TCA-NSI were 6.2%, 78.23%, 59.13%, 16.66%, and 81.06% respectively,and the dried product was white powder; by fluidized bed drying, the data were 11.14%, 22.49%, 5.04%, 2.03% and 28.99% respectively, and the dried product was yellow fine granular. Spray drying was more complicated to operate, time-consuming and expensive than fluidized bed drying, but it had higher effective glutamine. The two drying metheds could be used in different area for preparing wheat protein hydrolysate.