The total content of sterols in 4 kinds of special vegetable oils,i.e. peony seed oil, walnut oil, pumpkin seed oil and grape seed oil, and their composition were analyzed by gas chromatography. The result showed that sterols content in peony oil was 240.0 mg/100 g, of which the main components were β-sitosterol, Δ5, 23-stigmastadienol and Δ5-avenasterol; In walnut oil, the total content of sterols was 100.5 mg/100 g and β-sitosterol was the main component; In pumpkin seed oil,the total content of sterols was 299.6~358.4 mg/100 g, and the main components were β-sitosterol, Δ5,24-stigmastadienol and Δ7-avenasterol; In grape seed oil,the total content of sterols was 222.5~234.9 mg/100 g, and the main components were β-sitosterol, campesterol sterol and stigmasterol. The research provides basic data for development and process of special vegetable oil.