The edible safety of synthesized structured lipids（SLs）from rapeseed oil and octanoic acid by enzymic method was studied, in order to provide scientific basis for using it as a new resource of food. The toxicity of SLs was evaluated by acute toxicity test and heritage toxicity test of mice (Ames test, micronucleus test of bone marrow PCE cell in mice and sperm shape abnormality test of mice). The results showed that in the acute toxicity test, the oral maximum tolerance dose of SLs was larger than 20.46 g/kg bw, which meant the SLs belonging to non-toxic grade; The results of the heritage toxicity tests were all negative, suggested that there were no genetic toxicity in the structured lipids. The synthesized structured lipids（SLs）from rapeseed oil and octanoic acid by enzymic method belong to non-toxic grade food and do not have genetic toxic effect.