The Key Magazine of China Science and Technology
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Determination of acetoin in fermentation broth by high performance liquid chromatography
The acetoin microorganism fermentation system was prepared by biological method. The method to determine acetoin by high performance liquid chromatography was studied. The fermentation broth was pre-treated by centrifuging and filtering with 0.22 μm microporous membrane. A column of Hypersil NH2（300 mm×4.6 mm，5 μm）with an mobile phase containing acetonitrile and water(90∶10,V/V) was used for the analysis of acetoin in fermentation system, the flow rate was 1 mL/min, the column temperature and detector temperature was 35 ℃,the chromatography was completed in just 25 min. The recovery rate was 95.96%～101.2% and the relative standard deviations 1.69%～2.09%. The method was proved to be a rapid and effective quantitative analysis method with simple pretreatment and high sensitive to determine the acetoin in fermentation broth, which was fit for guiding real-time monitoring the fermentation process.