SCIENCE AND TECHNOLOGY OF CEREALS, OILS AND FOODS

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Determination of acetoin in fermentation broth by high performance liquid chromatography
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    Abstract:

    The acetoin microorganism fermentation system was prepared by biological method. The method to determine acetoin by high performance liquid chromatography was studied. The fermentation broth was pre-treated by centrifuging and filtering with 0.22 μm microporous membrane. A column of Hypersil NH2(300 mm×4.6 mm,5 μm)with an mobile phase containing acetonitrile and water(90∶10,V/V) was used for the analysis of acetoin in fermentation system, the flow rate was 1 mL/min, the column temperature and detector temperature was 35 ℃,the chromatography was completed in just 25 min. The recovery rate was 95.96%~101.2% and the relative standard deviations 1.69%~2.09%. The method was proved to be a rapid and effective quantitative analysis method with simple pretreatment and high sensitive to determine the acetoin in fermentation broth, which was fit for guiding real-time monitoring the fermentation process.

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History
  • Received:
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  • Online: February 17,2015
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