The wheat gluten protein polypeptide was prepared by compound enzyme method with wheat gluten powder as raw material. With the degree of hydrolysis(DH) of wheat gluten enzymatic hydrolysate, DPPH radical clearance rate and Fe3+ reducing ability as index, taking three levels of the four factors, the proportion of enzymes (alkaline protease∶wheat protein hydrolysis special enzyme), reaction time, reaction temperature and reaction pH, the optimal preparation conditions were determined by L9 (34) orthogonal test. The optimal technological conditions were as follows: the proportion of enzymes 2∶1, reaction time 2 h, reaction temperature 50 ℃, reaction pH 8.5; The verification experiments were conducted under the optimal conditions, resulting in DH of the enzymatic hydrolysate 29.9%, DPPH free radical clearance rate 89.9%, Fe3+ reducing ability 0.150.