SCIENCE AND TECHNOLOGY OF CEREALS, OILS AND FOODS

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Home > Archive>Volume 22, Issue 1, 2014 >82-84
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Study on determination of vitamin B1 in germ-retained rice and polished rice by high performance liquid chromatography
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    Abstract:

    The method to detect vitamin B1 in germ-retained rice and polished rice by HPLC-UV detector was researched. The samples were extracted by 0.1 mol/L hydrochloric acid for three times in ultrasonic cleanser, and the extract solution was measured after being concentrated. The chromatographic conditions were that detection at 246nm, mobile phase was methanol:sodium acetate(0.1 mol/L) =80:20,column temperature was 30 ℃ ,current velocity was 0.9 mL/min. The result showed that coefficient of recovery of this method was above 90%, all of the RSD was less than 5%, the detection limit (S/N=3) was 0.012 μg/mL. This method was fast, easy to operate with better repeatability.

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  • Online: March 15,2014
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