Taking the high quality soybean as raw material, short-term storage for 35 days, the influence of different moisture content (10.58%,15.23%,20.17%) and temperature (20 ℃,25 ℃,30 ℃) on the crude fat and crude protein content, the total number of mold, quality of oil and soybean meal in the soybean were investigated. The results showed that, under the condition of different temperature and moisture stored for 35 days, the crude fat and crude protein content in soybeans didn’t change obviously; but the high temperature and high moisture storage conditions could easily make soybean mildew, total number of mold increased rapidly with the highest number up to 1.09×105 CUF/g; at the same time, the acid value and peroxide value of soybean oil increased significantly, respectively up to 5.21 mgKOH/g and 3.70 mmol/kg, the content of unsaturated fatty acid decreased slightly, the highest range of reduction up to 5.01%; the KOH protein solubility of defatted soybean meal decreased obviously, the lowest down to 59.72%.