引用本文:李 慧,王殿轩.印度谷螟PintGOBP2基因的全长序列克隆及其生物信息学分析[J].粮油食品科技,2019,27(5):75-82.
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印度谷螟PintGOBP2基因的全长序列克隆及其生物信息学分析
李 慧, 王殿轩
河南工业大学 粮油食品学院,粮食储藏与安全教育部工程研究中心,粮食储运国家工程实验室,河南 郑州 450001
摘要:
印度谷螟Plodia interpunctella(Hübner),是一种危害性较严重的仓储害虫,在我国广泛分布。在其感染寄主的过程中,气味结合蛋白(OBPs)对印度谷螟的行为反应有重要作用。通过转录组数据筛选、鉴定获得印度谷螟OBP基因序列,并命名为PintGOBP2,用DNAMAN对其进行序列分析,BLAST进行同源性比较,并使用MEGA6.0构建系统发育进化树。结果表明:印度谷螟普通气味结合蛋白PintGOBP2的编码基因开放阅读框全长426 bp,编码141个氨基酸,预测分子量为15.48 kDa,等电点为4.39;N末端具有16个氨基酸残基组成的信号肽序列,并含有由6个半胱氨酸组成的保守片段,属于Classical OBPs亚家族基因。同源比对结果表明,PintGOBP2基因编码蛋白与其他鳞翅目昆虫GOBP蛋白具有较高的同源性。进化树分析结果表明,PintGOBP2基因与翅目昆虫GOBP超家族内相关基因的同源很高。研究结果为进一步分析印度谷螟PintGOBP2蛋白在其嗅觉信号传导过程中的生理功能提供了基础材料。
关键词:  印度谷螟  普通气味结合蛋白  基因克隆  生物信息学分析
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基金项目:
Cloning and bioinformatic analysis of PintGOBP2 gene in Plodiainterpunctella (Hübener)
Abstract:
The Indian meal moth, Plodiainterpunctella (Hübner) (Lepidoptera: Pyralidae, Pyralidae) is a kind of destructive polyphagous storage pests that is widely distributed in China. The odorant binding protein (OBPs) plays an important role in the behavioral response of the Indian moth in infection of its host. An Indian meal mothOBP gene sequence was obtained by screening and identifying the transcriptome dataand named PintGOBP2, which was analyzed by DNAMAN and homology comparison was performed by BLAST, phylogenetic trees were constructed using MEGA 6.0. The results showed that the open reading frame of the PintGOBP2 was 426 bp, encoding 141 amino acids with a molecular weight 15.48 kDa, and an isoelectric point of 4.39; the deduced amino acid sequence possesses a putative signal peptide of 16 amino acid residues at the N terminus. There are 6 conservative cysteine sites in the protein sequence, belongs to the Classical OBPs family genes. Homologous alignment showed that PintGOBP2 has high similarity with other lepidoptera insects GOBP. Further Phylogenetic analysis showed that PintGOBP2 could be clustered in the GOBP superfamily of lepidoptera insects. The results provide a molecular basis for further analysis of the physiological function of PintGOBP2 in the process of olfactory signal transmission in the P. interpunctella.
Key words:  Plodiainterpunctella (Hübner)  general odorant binding protein  gene cloning  bioinformatics analysis